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vector potb7 mmp1  (New England Biolabs)


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    Structured Review

    New England Biolabs vector potb7 mmp1
    (A) <t>MMP1</t> secretion level of stable MDCK- MMP1 colonies. (B) Cellular adhesion quantified by shear flow chamber assays. Significance: ** p-value < 0.03, * p-value > 0.05.
    Vector Potb7 Mmp1, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 647 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vector+potb7+mmp1/StuI/pmc04743853-41-2-11
    Average 96 stars, based on 647 article reviews
    vector potb7 mmp1 - by Bioz Stars, 2026-10
    96/100 stars

    Images

    1) Product Images from "Stable Ectopic Expression of ST6GALNAC5 Induces Autocrine MET Activation and Anchorage-Independence in MDCK Cells"

    Article Title: Stable Ectopic Expression of ST6GALNAC5 Induces Autocrine MET Activation and Anchorage-Independence in MDCK Cells

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0148075

    (A) MMP1 secretion level of stable MDCK- MMP1 colonies. (B) Cellular adhesion quantified by shear flow chamber assays. Significance: ** p-value < 0.03, * p-value > 0.05.
    Figure Legend Snippet: (A) MMP1 secretion level of stable MDCK- MMP1 colonies. (B) Cellular adhesion quantified by shear flow chamber assays. Significance: ** p-value < 0.03, * p-value > 0.05.

    Techniques Used:

    Diagrams are simplified based on known mechanisms. (A) Hyposialyation leads to diminished response of the receptor and thus no pathway activation. (B) Sufficient sialylation on MET allows activation by its ligand, HGF, causing up-regulation of MMP1 and down-regulation of cell adhesion molecules.
    Figure Legend Snippet: Diagrams are simplified based on known mechanisms. (A) Hyposialyation leads to diminished response of the receptor and thus no pathway activation. (B) Sufficient sialylation on MET allows activation by its ligand, HGF, causing up-regulation of MMP1 and down-regulation of cell adhesion molecules.

    Techniques Used: Activation Assay

    Related Articles

    Amplification:

    Article Title: Stable Ectopic Expression of ST6GALNAC5 Induces Autocrine MET Activation and Anchorage-Independence in MDCK Cells
    Article Snippet: The pOTB7- mmp1 vector was transformed in a competent E. coli BL21 strain (F - ompT hsdS B (r B - m B - ) gal dcm , Novagen) to eliminate dcm methylation on plasmid before digestion. .. The amplified vector pOTB7-MMP1 was digested using StuI and XhoI enzymes (New England Biolabs) at 37°C for 2 h to release the gene insert. .. The cDNA was then purified using the QIAquick gel extraction kit (Qiagen) after separation by 1% agarose TBE gel electrophoresis.



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    (A) <t>MMP1</t> secretion level of stable MDCK- MMP1 colonies. (B) Cellular adhesion quantified by shear flow chamber assays. Significance: ** p-value < 0.03, * p-value > 0.05.
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    vector potb7 mmp1  (New England Biolabs)
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    (A) <t>MMP1</t> secretion level of stable MDCK- MMP1 colonies. (B) Cellular adhesion quantified by shear flow chamber assays. Significance: ** p-value < 0.03, * p-value > 0.05.
    Vector Potb7 Mmp1, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vector+potb7+mmp1/StuI/pmc04743853-41-2-11
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    Image Search Results


    (A) MMP1 secretion level of stable MDCK- MMP1 colonies. (B) Cellular adhesion quantified by shear flow chamber assays. Significance: ** p-value < 0.03, * p-value > 0.05.

    Journal: PLoS ONE

    Article Title: Stable Ectopic Expression of ST6GALNAC5 Induces Autocrine MET Activation and Anchorage-Independence in MDCK Cells

    doi: 10.1371/journal.pone.0148075

    Figure Lengend Snippet: (A) MMP1 secretion level of stable MDCK- MMP1 colonies. (B) Cellular adhesion quantified by shear flow chamber assays. Significance: ** p-value < 0.03, * p-value > 0.05.

    Article Snippet: The pOTB7- mmp1 vector was transformed in a competent E. coli BL21 strain (F - ompT hsdS B (r B - m B - ) gal dcm , Novagen) to eliminate dcm methylation on plasmid before digestion.

    Techniques:

    Diagrams are simplified based on known mechanisms. (A) Hyposialyation leads to diminished response of the receptor and thus no pathway activation. (B) Sufficient sialylation on MET allows activation by its ligand, HGF, causing up-regulation of MMP1 and down-regulation of cell adhesion molecules.

    Journal: PLoS ONE

    Article Title: Stable Ectopic Expression of ST6GALNAC5 Induces Autocrine MET Activation and Anchorage-Independence in MDCK Cells

    doi: 10.1371/journal.pone.0148075

    Figure Lengend Snippet: Diagrams are simplified based on known mechanisms. (A) Hyposialyation leads to diminished response of the receptor and thus no pathway activation. (B) Sufficient sialylation on MET allows activation by its ligand, HGF, causing up-regulation of MMP1 and down-regulation of cell adhesion molecules.

    Article Snippet: The pOTB7- mmp1 vector was transformed in a competent E. coli BL21 strain (F - ompT hsdS B (r B - m B - ) gal dcm , Novagen) to eliminate dcm methylation on plasmid before digestion.

    Techniques: Activation Assay

    (A) MMP1 secretion level of stable MDCK- MMP1 colonies. (B) Cellular adhesion quantified by shear flow chamber assays. Significance: ** p-value < 0.03, * p-value > 0.05.

    Journal: PLoS ONE

    Article Title: Stable Ectopic Expression of ST6GALNAC5 Induces Autocrine MET Activation and Anchorage-Independence in MDCK Cells

    doi: 10.1371/journal.pone.0148075

    Figure Lengend Snippet: (A) MMP1 secretion level of stable MDCK- MMP1 colonies. (B) Cellular adhesion quantified by shear flow chamber assays. Significance: ** p-value < 0.03, * p-value > 0.05.

    Article Snippet: The amplified vector pOTB7-MMP1 was digested using StuI and XhoI enzymes (New England Biolabs) at 37°C for 2 h to release the gene insert.

    Techniques:

    Diagrams are simplified based on known mechanisms. (A) Hyposialyation leads to diminished response of the receptor and thus no pathway activation. (B) Sufficient sialylation on MET allows activation by its ligand, HGF, causing up-regulation of MMP1 and down-regulation of cell adhesion molecules.

    Journal: PLoS ONE

    Article Title: Stable Ectopic Expression of ST6GALNAC5 Induces Autocrine MET Activation and Anchorage-Independence in MDCK Cells

    doi: 10.1371/journal.pone.0148075

    Figure Lengend Snippet: Diagrams are simplified based on known mechanisms. (A) Hyposialyation leads to diminished response of the receptor and thus no pathway activation. (B) Sufficient sialylation on MET allows activation by its ligand, HGF, causing up-regulation of MMP1 and down-regulation of cell adhesion molecules.

    Article Snippet: The amplified vector pOTB7-MMP1 was digested using StuI and XhoI enzymes (New England Biolabs) at 37°C for 2 h to release the gene insert.

    Techniques: Activation Assay